Judith Butler’s Gender Trouble (1990) has been called the single most important work in shaping the development of queer and feminist theory in recent decades. Celebrating the 25th anniversary of Gender Trouble, this special issue of lambda nordica specifically takes stock of some of the impact it has had in Nordic queer and feminist studies. We present work by scholars from different fields and academic generations and with different takes on the significance of Judith Butler’s interventions and contributions. With four articles and three essays, this double issue thus offers both theoretical insights and personal reflections. Some shed new light on themes from Gender Trouble such as performativity and the question of ontology, while others show the impact and uses of Butler’s ideas and concepts on both academic and activist practices.